primary human hair follicle dermal papilla cells hfdpc  (Cell Applications Inc)

Journal: Viruses

Article Title: Assessment of Prolonged Dengue Virus Infection in Dermal Fibroblasts and Hair-Follicle Dermal Papilla Cells

doi: 10.3390/v12030267

Figure Lengend Snippet: Analysis of the prolonged infection of DENV-2 in hair-follicle dermal papilla cells (HFDPCs). ( A ) HFDPCs (4 × 10 4 ) were infected with mock or DENV-2 MOI 10 for four days; an immunofluorescence assay was conducted to detect DENV-2 NS3 (green). The images merged with DAPI staining of cell nuclei (blue) are also shown, scale bar: 100 μm. ( B ) DENV-2-infected cells were detected by an immunofluorescence assay in DENV-2 (MOI 1, 5, and 10)-infected HFDPCs cells after prolonged infection for 33 days. ( C ) DENV-2 infectivity at day 33 post-infection was calculated. ( D ) The intensity shows the fluorescence ratio of NS3 vs. DAPI in post-infected cells on day 33. ( E ) The morphologies of DENV-2 (MOI 1, 5, and 10)-infected HFDPCs were obtained by phase contrast light microscopy at days 1, 2, and 33 post-infection. The arrows indicate the spots of CPE. ( F ) The culture medium was harvested on day 1, 2, and 33 post-infection for the LDH cell cytotoxicity assay. ( G ) qRT-PCR analysis of mRNA expression of caspase 3 ( CAS3 , upper panel) and caspase 7 ( CAS7 , lower panel) in HFDPCs with DENV-2 (MOI 1, 5 and 10) infection. Relative mRNA expression normalized to that of Gapdh and the fold induction to mock control. Data are presented as means ± SD ( n = 3). Student’s t -test *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with mock infection groups.

Article Snippet: Primary human hair-follicle dermal papilla cells (HFDPCs) were isolated from the hair papilla of normal human scalp hair follicles (Cat. 602t-05a, Cell Applications, Inc. San Diego, CA, USA).

Techniques: Infection, Immunofluorescence, Staining, Fluorescence, Light Microscopy, Cytotoxicity Assay, Quantitative RT-PCR, Expressing, Control

Journal: Viruses

Article Title: Assessment of Prolonged Dengue Virus Infection in Dermal Fibroblasts and Hair-Follicle Dermal Papilla Cells

doi: 10.3390/v12030267

Figure Lengend Snippet: Evaluation of the DENV-2 replication in HFDPCs after prolonged infection. ( A ) The DENV-2 RNA (5′-UTR) replication was detected by qRT-PCR in HFDPCs (4 × 10 4 ) with DENV-2 (MOI 1, 5, and 10) after 1, 2, and 33 days. The expression level of DENV-2 RNA was normalized to Gapdh , and the fold induction to mock control. Data are presented as mean ± SD from three independent tests. ( B ) The plaque assay was conducted with the culture medium of DENV-2-infected HFDPCs (dilution factor: 10 −1 to 10 −6 ). ( C ) The virus yield by HFDPCs from the plaque assay are presented in log 10 plaque-forming units per milliliter (PFU/mL) from three independent assays. ( D ) The diameter of the plaque size in the plaque assay. Data are presented as mean ± SD, * p < 0.05, *** p < 0.001, *** p < 0.005 vs. mock control.

Article Snippet: Primary human hair-follicle dermal papilla cells (HFDPCs) were isolated from the hair papilla of normal human scalp hair follicles (Cat. 602t-05a, Cell Applications, Inc. San Diego, CA, USA).

Techniques: Infection, Quantitative RT-PCR, Expressing, Control, Plaque Assay, Virus

Journal: Viruses

Article Title: Assessment of Prolonged Dengue Virus Infection in Dermal Fibroblasts and Hair-Follicle Dermal Papilla Cells

doi: 10.3390/v12030267

Figure Lengend Snippet: Analysis of the anti-viral inflammation in HFDPCs with acute or long-term DENV-2 infections. ( A ) qRT-PCR of the indicated mRNA expression in WS1 cells infected with DENV-2 at MOI 1, 5, and 10 for 1, 2, and 33 days. The gene expression was normalized to Gapdh . Data are presented as mean ± SD from three independent tests, * p < 0.05, ** p < 0.01, *** p < 0.005 vs. mock control. ( B ) The DENV-2-infected HFDPCs lysates were subjected to SDS-PAGE and Western blot analysis. Representative data were obtained from three independent tests. Quantification of each protein level at days 1, 2, and 33 days with GAPDH was normalized to individual mock control.

Article Snippet: Primary human hair-follicle dermal papilla cells (HFDPCs) were isolated from the hair papilla of normal human scalp hair follicles (Cat. 602t-05a, Cell Applications, Inc. San Diego, CA, USA).

Techniques: Quantitative RT-PCR, Expressing, Infection, Gene Expression, Control, SDS Page, Western Blot

Journal: Viruses

Article Title: Assessment of Prolonged Dengue Virus Infection in Dermal Fibroblasts and Hair-Follicle Dermal Papilla Cells

doi: 10.3390/v12030267

Figure Lengend Snippet: The expression of hair growth cycle genes in cells with DENV-2 infection for indicated days. ( A , B ) qRT-PCR of Rip-1, Wnt1, Wnt4 and cMyc was conducted in WS1 and HFDPCs with DENV-2 (MOI 1, 5, and 10) for 1, 2, and 33 days. The mRNA expression level was normalized to Gapdh , and the fold induction to the mock control. Data are presented as mean ± SD from three independent tests. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. mock control.

Article Snippet: Primary human hair-follicle dermal papilla cells (HFDPCs) were isolated from the hair papilla of normal human scalp hair follicles (Cat. 602t-05a, Cell Applications, Inc. San Diego, CA, USA).

Techniques: Expressing, Infection, Quantitative RT-PCR, Control